AquatiqPCR©-C/G

AquatiqPCR©-C/G Molecular detection of pathogenic Cryptosporidium and Giardia in water Advantages:

  • No need for an expert eye – Dismiss microscopic examination
  • Fast – Definitive results are achieved in a matter of hours
  • Sensitive – Less than 5 oocysts/cysts per sample (filtrate of 50l)
  • Specific – Prevalent pathogenic species only*
  • Robust – Excellent reproducibility on any qPCR instrument 
  • Cost effective
    • A lab can process tens to hundreds samples a day as opposed to today’s routine of only a few per day
    • Low TCO (Total Cost of Ownership) is a fraction of the cost of current tests
    • Decreases manpower costs – Can be operated by any molecular biologist

AquatiqPCR©-C/G** 30-001 (24 samples) Multiplex, qPCR water testing kit for the simultaneous detection of pathogenic Cryptosporidium and Giardia in the same tube. AquatiqPCR©-C** 30-002 (24 samples) Singleplex qPCR detection of pathogenic Cryptosporidium AquatiqPCR©-G** 30-003 (24 samples) Singleplex qPCR detection of pathogenic Giardia * For research use only ** C. parvum and C. hominis and G. lamblia assemblages A&B. Assay specificity The assay is specific for pathogenic Cryptosporidium species and Giardia assemblages A&B. Other related protozoa that were tested (e.g., Entamoeba histolitica, Babesia canis, Babesia gibsoni, Theileria annae, Toxoplasma gondii and Trypanosoma brucei) showed negative results under the same conditions. CG_Graph Assay Quantification The above is a typical standard plot of serial decimal dilutions of target Cryptosporidium and Giardia DNA sequences (green dots). Blue dots indicate water samples containing 30 and 3 Cysts/Oocysts, respectively. Environmental water samples were spiked with Cysts and Oocysts from validated spiking stocks (Waterborne, Inc.) and tested with AquatiqPCR©-C/G kit. Results The number of Giardia Cysts and Cryptosporidium Oocytes can be deduced by a standard plot based on serial decimal dilutions of target DNA oligo’s. Taking into account that every Giardia Cyst contains 8 sets of genomic DNA and each Cryptosporidium Oocyst contains 4 sets, we are able to translate the DNA copy number from the qPCR reaction into actual numbers of Cyst/Oocyte.

Tested succesfully by Tetra Tech, Clancy Environmental Lab.
in three different water sources in the US.

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AquatiqPCR©-C/G vs. USEPA Method 1623 in environmental water filtrate. A side-by-side study was conducted at the Central Lab of Mekorot Ltd. (Israel National Water Company). Filtrates of 50 liter environmental water samples were spiked with 5 or 10 Giardia cysts and 5 or 10 Cryptosporidium oocytes, respectively. Results Numbers above the columns indicates the ΔCq values as per the qPCR analysis using AquatiqPCR©-C/G kit. Numbers underneath the columns shows the results of USEPA method 1623 (number of oocysts/cysts after extraction, immunostaining and microscopic examination). AquatiqPCR©-C/G kit was able to detected Cryptosporidium and Giardia DNA in all samples tested whereas the microscopic examination was inaccurate and failed to detect Cryptosporidium in low levels (false negative).