AquatiqPCR©-C/G Molecular detection of pathogenic Cryptosporidium and Giardia in water Advantages:
AquatiqPCR©-C/G** 30-001 (24 samples) Multiplex, qPCR water testing kit for the simultaneous detection of pathogenic Cryptosporidium and Giardia in the same tube. AquatiqPCR©-C** 30-002 (24 samples) Singleplex qPCR detection of pathogenic Cryptosporidium AquatiqPCR©-G** 30-003 (24 samples) Singleplex qPCR detection of pathogenic Giardia * For research use only ** C. parvum and C. hominis and G. lamblia assemblages A&B. Assay specificity The assay is specific for pathogenic Cryptosporidium species and Giardia assemblages A&B. Other related protozoa that were tested (e.g., Entamoeba histolitica, Babesia canis, Babesia gibsoni, Theileria annae, Toxoplasma gondii and Trypanosoma brucei) showed negative results under the same conditions. Assay Quantification The above is a typical standard plot of serial decimal dilutions of target Cryptosporidium and Giardia DNA sequences (green dots). Blue dots indicate water samples containing 30 and 3 Cysts/Oocysts, respectively. Environmental water samples were spiked with Cysts and Oocysts from validated spiking stocks (Waterborne, Inc.) and tested with AquatiqPCR©-C/G kit. Results The number of Giardia Cysts and Cryptosporidium Oocytes can be deduced by a standard plot based on serial decimal dilutions of target DNA oligo’s. Taking into account that every Giardia Cyst contains 8 sets of genomic DNA and each Cryptosporidium Oocyst contains 4 sets, we are able to translate the DNA copy number from the qPCR reaction into actual numbers of Cyst/Oocyte.
Tested succesfully by Tetra Tech, Clancy Environmental Lab.
in three different water sources in the US.
AquatiqPCR©-C/G vs. USEPA Method 1623 in environmental water filtrate. A side-by-side study was conducted at the Central Lab of Mekorot Ltd. (Israel National Water Company). Filtrates of 50 liter environmental water samples were spiked with 5 or 10 Giardia cysts and 5 or 10 Cryptosporidium oocytes, respectively. Results Numbers above the columns indicates the ΔCq values as per the qPCR analysis using AquatiqPCR©-C/G kit. Numbers underneath the columns shows the results of USEPA method 1623 (number of oocysts/cysts after extraction, immunostaining and microscopic examination). AquatiqPCR©-C/G kit was able to detected Cryptosporidium and Giardia DNA in all samples tested whereas the microscopic examination was inaccurate and failed to detect Cryptosporidium in low levels (false negative).